TY - JOUR T1 - Arachidonic acid metabolism controls macrophage alternative activation through regulating oxidative phosphorylation in PPARG dependent manner JF - bioRxiv DO - 10.1101/2020.10.15.340265 SP - 2020.10.15.340265 AU - Miao Xu AU - Xiaohong Wang AU - Xudong Jia AU - Yongning Li AU - Xue Geng AU - Lishi Zhang AU - Hui Yang Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/10/15/2020.10.15.340265.abstract N2 - Macrophages polarization is mainly controlled by metabolic reprogramming in microenvironment, thus leading to distinct outcomes of various diseases. However, the role of lipid metabolism in the regulation of macrophage alternative activation is incompletely understood. Using human THP-1 and mouse bone marrow derived macrophages polarization models, we revealed a pivotal role for arachidonic acid metabolism in controlling the polarization of M1 and M2 macrophages. We demonstrated that M2 macrophage polarization was inhibited by arachidonic acid, but inversely facilitated by its derived metabolite prostaglandin E2 (PGE2). Furthermore, PPARG bridges these two unconnected processes via modulating oxidative phosphorylation. These results highlight the critical role of arachidonic acid metabolism as an immune regulator in modulating metabolic homeostasis and pathological process.Competing Interest StatementThe authors have declared no competing interest. ER -