RT Journal Article
SR Electronic
T1 The Effects of Inter-Individual Biological Differences and Taphonomic Alteration on Human Bone Protein Profiles: Implications for the Development of PMI/AAD Estimation Methods
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.10.15.341156
DO 10.1101/2020.10.15.341156
A1 Hayley L. Mickleburgh
A1 Ed Schwalbe
A1 Haruka Mizukami
A1 Federica Sellitto
A1 Sefora Starace
A1 Daniel J. Wescott
A1 David O. Carter
A1 Noemi Procopio
YR 2020
UL http://biorxiv.org/content/early/2020/10/15/2020.10.15.341156.abstract
AB Bone proteomics studies using animal proxies and skeletonized human remains have delivered encouraging results in the search for potential biomarkers for precise and accurate post-mortem interval (PMI) and the age-at-death (AAD) estimation in medico-legal investigations. At present, however, the effects of inter-individual biological differences and taphonomic alteration on recovered human bone protein profiles are not well understood. This study investigated the human bone proteome in four human body donors studied throughout decomposition outdoors. The effects of ageing phenomena (in vivo and post-mortem), and intrinsic and extrinsic variables on the variety and abundancy of the bone proteome were assessed. Results identified a new potential biomarker for PMI estimation, as well as three potential biomarkers for AAD estimation. The results also suggest that bone mineral density (BMD) may be an important variable affecting the survival and extraction of proteins.Highlights- CO3, CO9, COBA2, CO3A1, MGP, PGS2 and TTHY are potential biomarkers for post-mortem interval estimation in skeletonized human remains- FETUA, ALBU and OLFL3 are potential biomarkers for age-at-death estimation in human remains- Taphonomic and biological variables play a significant role in survival and extraction rates of proteins in bone- Bone mineral density may affect survival of proteins in bone, probably due to the effects of the mineral matrix on the movement of decomposer microbes- Higher bone mineral density may affect the survival and the extraction rate of collagen and mineral-binding proteinsCompeting Interest StatementThe authors have declared no competing interest.