PT - JOURNAL ARTICLE AU - Yong-An Tan AU - Xu-Dong Zhao AU - Jing Zhao AU - Qin-Qin Ji AU - Liu-Bin Xiao AU - De-Jun Hao TI - iTRAQ-Based Proteomic Profiling of PLC-Mediated Expression of 20E-Induced Protein in <em>Apolygus lucorum</em> (Meyer-Dür) AID - 10.1101/2020.10.15.341115 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.10.15.341115 4099 - http://biorxiv.org/content/early/2020/10/15/2020.10.15.341115.short 4100 - http://biorxiv.org/content/early/2020/10/15/2020.10.15.341115.full AB - The polyphagous pest Apolygus lucorum has become the dominant insect in Bacillus thuringiensis (Bt) cotton fields. The hormone 20-hydroxyecdysone (20E) regulates multiple events in insect development and physiology. 20E responses are controlled by pathways triggered by phospholipase C (PLC)-associated proteins. However, 20E-modulated genes whose expression is affected by PLC remain unknown. Here, isobaric tag for relative and absolute quantitation (iTRAQ) and immunoblot were carried out for comparing differentially expressed proteins (DEPs) in A. lucorum in response to 20E and the PLC inhibitor U73122, respectively. Totally 1624 DEPs were, respectively, found in the 20E/control, U73122/control, and 20E+U73122/control groups. Venn diagram analysis further revealed 8 DEPs that were shared among the three groups. Immunoblot validated these findings, which corroborated and highlighted the reliability of proteomics. KEGG enrichment analysis showed that the DEPs were included in diverse signaling pathways. The largest portion of DEPs among the three groups were categorized in metabolic pathways. In addition, DEPs among the three groups were also found to regulate the Ras-MAPK and PI3K-AKT pathways. This is the first time that iTRAQ was carried out to assess proteome alteration in A. lucorum nymphs in response to 20E and a PLC inhibitor. These findings provide novel insights into protein expression in A. lucorum in response to 20E, and a more comprehensive understanding of the function of PLC in 20E signal transduction.