PT  - JOURNAL ARTICLE
AU  - Claire Lamaison
AU  - Simon Latour
AU  - Nelson Hélaine
AU  - Valérie Le Morvan
AU  - Céline Monvoisin
AU  - Isabelle Mahouche
AU  - Christelle Dussert
AU  - Elise Dessauge
AU  - Céline Pangault
AU  - Marine Seffals
AU  - Léa Broca-Brisson
AU  - Kévin Alessandri
AU  - Pierre Soubeyran
AU  - Frédéric Mourcin
AU  - Pierre Nassoy
AU  - Gaëlle Recher
AU  - Karin Tarte
AU  - Laurence Bresson-Bepoldin
TI  - Stromal cells regulate malignant B-cell spatial organization, survival, and drug response in a new 3D model mimicking lymphoma tumor niche
AID  - 10.1101/2020.10.17.343657
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.10.17.343657
4099  - http://biorxiv.org/content/early/2020/10/17/2020.10.17.343657.short
4100  - http://biorxiv.org/content/early/2020/10/17/2020.10.17.343657.full
AB  - Non-Hodgkin B-cell lymphomas (B-NHL) mainly develop within lymph nodes as densely packed aggregates of tumor cells and their surrounding microenvironment, creating a tumor niche specific to each lymphoma subtypes. Until now, in vitro preclinical models mimicking biomechanical forces, cellular microenvironment, and 3D organization of B lymphomas remain scarce while all these parameters constitute key determinants of lymphomagenesis and drug resistance. Using a microfluidic method based on the encapsulation of cells inside permeable, elastic, and hollow alginate microspheres, we developed a new tunable 3D-model incorporating extracellular matrix and/or stromal cells. Lymphoma B cells and stromal cells dynamically formed self-organized 3D spheroids, thus initiating a coevolution of these two cell types, reflecting their bidirectional crosstalk, and recapitulating the heterogeneity of B-NHL subtypes. In addition, this approach makes it suitable to assess in a relevant in vitro model the activity of new therapeutic agents in B-NHL.Competing Interest StatementThe authors have declared no competing interest.