RT Journal Article
SR Electronic
T1 Mitochondrial fission governed by Drp1 regulates exogenous fatty acid usage and storage
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.10.17.343616
DO 10.1101/2020.10.17.343616
A1 Jae Eun Song
A1 Tiago Alves
A1 Bernardo Stutz
A1 Matija Sestan-Pesa
A1 Nicole Kilian
A1 Sabrina Diano
A1 Richard G. Kibbey
A1 Tamas L. Horvath
YR 2020
UL http://biorxiv.org/content/early/2020/10/17/2020.10.17.343616.abstract
AB The bioenergetic function of mitochondrial fission is associated with uncoupled respiration or elimination of damaged mitochondria to maintain a healthy mitochondrial population. In the presence of a high abundance of exogenous fatty acids, cells can either store fatty acids in lipid droplets or oxidize them in mitochondria. Even though carnitine palmitoyltransferase-1 (CPT1) controls the respiratory capacity of mitochondria on fatty acid oxidation, we observed in HeLa cells that it does not dictate the balance of storage and usage of lipids. On the other hand, inhibition of mitochondrial fission by silencing dynamic-related protein 1 (DRP1) resulted in an increase in fatty acid content of lipid droplets and a decrease in fatty acid oxidation. Mitochondrial fission was not only reflective of the amount of exogenous fatty acid being processed by mitochondria but it was also found to be actively involved in the distribution of fatty acids between mitochondria and lipid droplets. Our data we revealed a novel function for mitochondrial fission in balancing exogenous fatty acids between usage and storage, assigning a role for mitochondrial dynamics in control of intracellular fuel utilization and partitioning.Competing Interest StatementThe authors have declared no competing interest.