TY - JOUR T1 - Derivation of human extended pluripotent stem cells in feeder-free condition JF - bioRxiv DO - 10.1101/2020.10.18.344259 SP - 2020.10.18.344259 AU - Ran Zheng AU - Ting Geng AU - Dan-Ya Wu AU - Tianzhe Zhang AU - Hai-Nan He AU - Liyan Wang AU - Haining Du AU - Donghui Zhang AU - Yi-Liang Miao AU - Wei Jiang Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/10/18/2020.10.18.344259.abstract N2 - Human extended pluripotent stem cells (EPSCs), with bidirectional chimeric ability to contribute to both embryonic and extra-embryonic lineages, can be obtained and maintained by converting embryonic stem cells (ESCs) using chemicals. However, the transition system is based on inactivated mouse fibroblast, which greatly hinders the mechanistic studies of extended pluripotency and further applications. Here we reported a Matrigel-based feeder-free method to convert human ESCs and iPSCs into EPSCs and demonstrated the extended pluripotency in terms of molecular features, chimeric ability, and transcriptome. We further improved the protocol by applying chemicals targeting glycolysis and histone methyltransferase. Altogether, our data established a feeder-free system to generate human EPSCs and provided additional insights into the acquisition of extended pluripotency.Competing Interest StatementThe authors have declared no competing interest. ER -