TY - JOUR T1 - Direct visualization of epithelial microvilli biogenesis JF - bioRxiv DO - 10.1101/2020.10.21.341248 SP - 2020.10.21.341248 AU - Isabella M. Gaeta AU - Leslie M. Meenderink AU - Meagan M. Postema AU - Caroline S. Cencer AU - Matthew J. Tyska Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/10/21/2020.10.21.341248.abstract N2 - Microvilli are actin bundle supported surface protrusions that play essential roles in diverse epithelial cell functions. To develop our understanding of microvilli biogenesis, we used live imaging to directly visualize protrusion growth at early stages of epithelial differentiation. Time-lapse data revealed that an “initiation complex” enriched in EPS8 and IRTKS appears at future sites of microvillus growth minutes before core actin bundle assembly. Elongation of a new core bundle occurs in parallel with the arrival of EZRIN and plasma membrane encapsulation. In addition to de novo growth, we also observed that new microvilli emerge from pre-existing protrusions. Additionally, we found that new microvilli can also collapse, characterized first by loss of membrane wrapping and Ezrin enrichment, followed by a sharp decrease in distal tip EPS8 and IRTKS. These studies are the first to offer a temporally resolved microvillus growth mechanism and highlight critical factors that drive this process.Competing Interest StatementThe authors have declared no competing interest. ER -