RT Journal Article
SR Electronic
T1 Transcriptional Diversity of Medium Spiny Neurons in the Primate Striatum
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.10.25.354159
DO 10.1101/2020.10.25.354159
A1 Jing He
A1 Michael Kleyman
A1 Jianjiao Chen
A1 Aydin Alikaya
A1 Kathryn M. Rothenhoefer
A1 Bilge Esin Ozturk
A1 Morgan Wirthlin
A1 Kenneth Fish
A1 Leah C.T. Byrne
A1 Andreas R. Pfenning
A1 William R. Stauffer
YR 2020
UL http://biorxiv.org/content/early/2020/10/25/2020.10.25.354159.abstract
AB The striatum is the neural interface between dopamine reward signals and cortico-basal ganglia circuits responsible for value assignments, decisions, and actions. Medium spiny neurons (MSNs) make up the vast majority of striatal neurons and are traditionally classified as two distinct types: direct- and indirect-pathway MSNs. The direct- and indirect-pathway model has been useful for understanding some aspects of striatal functions, but it accounts for neither the anatomical heterogeneity, nor the functional diversity of the striatum. Here, we use single nucleus RNA-sequencing and Fluorescent In-Situ Hybridization to explore MSN diversity in the Rhesus macaque striatum. We identified MSN subtypes that correspond to the major subdivisions of the striatum. These include dorsal striatum subtypes associated with striosome and matrix compartments, as well as ventral striatum subtypes associated with the shell of the nucleus accumbens. We also describe a cell type that is anatomically restricted to “Neurochemically Unique Domains in the Accumbens and Putamen (NUDAPs)”. Together, these results help to advance nonhuman primate studies into the genomics era. The identified cell types provide a comprehensive blueprint for investigating cell type-specific information processing, and the differentially expressed genes lay a foundation for achieving cell type-specific transgenesis in the primate striatum.Competing Interest StatementThe authors have declared no competing interest.