RT Journal Article
SR Electronic
T1 Real-Time Multi-Angle Projection Imaging of Biological Dynamics
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.10.29.355917
DO 10.1101/2020.10.29.355917
A1 Bo-Jui Chang
A1 Etai Sapoznik
A1 Theresa Pohlkamp
A1 Tamara S. Terrones
A1 Erik S. Welf
A1 James D. Manton
A1 Philippe Roudot
A1 Kayley Hake
A1 Lachlan Whitehead
A1 Andrew G. York
A1 Kevin M. Dean
A1 Reto Fiolka
YR 2020
UL http://biorxiv.org/content/early/2020/10/29/2020.10.29.355917.abstract
AB We introduce a cost-effective and easily implemented scan unit which enables any camera-based microscope to perform projection imaging from diverse viewing angles. We demonstrate this capability on Lattice Light-Sheet and Oblique Plane Microscopy by rapidly delivering projection images with an uncompromised lateral resolution and high optical contrast. By imaging the sample from one or multiple perspectives, our method enables visualization of rapid biological processes, real time stereoscopic imaging as well as three-dimensional particle localization throughout a cellular volume from just two images. Furthermore, because our projection imaging technique provides intuitive three-dimensional renderings in real-time, it improves microscope usability, allows users to more-readily optimize instrument performance and identify biological phenomena of interest on-the-fly, while also reducing data overhead by a factor of >100. We leverage our rapid projection method to image cancer cell morpho-dynamics and calcium signaling in cultured neurons, to perform three-dimensional localization of genetically encoded nanoparticles, as well as to image orthogonal views of an embryonic Zebrafish heart simultaneously.Competing Interest StatementR.F. filed a patent for the scan unit and its applications to microscopy. K.M.D. and R.F. have an investment interest in Discovery Imaging Systems, LLC.