TY - JOUR T1 - Real-Time Multi-Angle Projection Imaging of Biological Dynamics JF - bioRxiv DO - 10.1101/2020.10.29.355917 SP - 2020.10.29.355917 AU - Bo-Jui Chang AU - Etai Sapoznik AU - Theresa Pohlkamp AU - Tamara S. Terrones AU - Erik S. Welf AU - James D. Manton AU - Philippe Roudot AU - Kayley Hake AU - Lachlan Whitehead AU - Andrew G. York AU - Kevin M. Dean AU - Reto Fiolka Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/10/29/2020.10.29.355917.abstract N2 - We introduce a cost-effective and easily implemented scan unit which enables any camera-based microscope to perform projection imaging from diverse viewing angles. We demonstrate this capability on Lattice Light-Sheet and Oblique Plane Microscopy by rapidly delivering projection images with an uncompromised lateral resolution and high optical contrast. By imaging the sample from one or multiple perspectives, our method enables visualization of rapid biological processes, real time stereoscopic imaging as well as three-dimensional particle localization throughout a cellular volume from just two images. Furthermore, because our projection imaging technique provides intuitive three-dimensional renderings in real-time, it improves microscope usability, allows users to more-readily optimize instrument performance and identify biological phenomena of interest on-the-fly, while also reducing data overhead by a factor of >100. We leverage our rapid projection method to image cancer cell morpho-dynamics and calcium signaling in cultured neurons, to perform three-dimensional localization of genetically encoded nanoparticles, as well as to image orthogonal views of an embryonic Zebrafish heart simultaneously.Competing Interest StatementR.F. filed a patent for the scan unit and its applications to microscopy. K.M.D. and R.F. have an investment interest in Discovery Imaging Systems, LLC. ER -