PT  - JOURNAL ARTICLE
AU  - Damiana Téllez-Martínez
AU  - Alexander Batista-Duharte
AU  - Vinicius Paschoalini Silva
AU  - Deivys Portuondo Fuentes
AU  - Lucas Souza Ferreira
AU  - Marisa Campos Polesi
AU  - Caroline Barcelós Costa
AU  - Iracilda Zeppone Carlos
TI  - Adaptive stress response induced by toluene increases <em>Sporothrix schenckii</em> virulence and host immune response
AID  - 10.1101/539775
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 539775
4099  - http://biorxiv.org/content/early/2019/02/04/539775.short
4100  - http://biorxiv.org/content/early/2019/02/04/539775.full
AB  - Environmental factors modify the physiology of microorganisms, allowing their survival in extreme conditions. However, the influence of chemical contaminants on fungal virulence has been little studied. Sporotrichosis is an emergent fungal disease caused by Sporothrix schenckii, a soil-inhabiting fungus that has been found in polluted environments. Here, we evaluated the adaptive stress response of S. schenckii induced by toluene, a key soil contaminant. The effect on fungal virulence and host immune response was also assessed. The fungus survived up to 0.10% toluene in liquid medium. Greater production of melanosomes and enhanced activity superoxide dismutase, associated to increased tolerance to H2O2 were observed in toluene-exposed fungi. Intraperitoneal infection of mice with S. schenckii treated with either 0, 0.01 or 0.1% of toluene, resulted in greater fungal burden at day 7 post-infection in spleen and liver in the groups infected with fungus treated with toluene 0.1%. A higher production of Il-1β, TNF-α, IL-10 and nitric oxyde by peritoneal macrophages and IFNγ, IL-4 and IL-17 by splenocytes was also observed in that group. Our findings showed that morphological and functional changes induced by toluene leads to increased S. schenckii virulence and antifungal host immune response in our model.