PT  - JOURNAL ARTICLE
AU  - Michael J Harris
AU  - Muna Fuyal
AU  - John R James
TI  - Quantifying signal persistence in the T cell signaling network using an optically controllable antigen receptor
AID  - 10.1101/2020.10.30.362194
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.10.30.362194
4099  - http://biorxiv.org/content/early/2020/10/30/2020.10.30.362194.short
4100  - http://biorxiv.org/content/early/2020/10/30/2020.10.30.362194.full
AB  - T cells discriminate between healthy and infected cells with remarkable sensitivity when mounting an immune response. It has been hypothesized that this efficient detection requires combining signals from discrete antigen-presenting cell interactions into a more potent response, requiring T cells to maintain a ‘memory’ of previous encounters. To quantify the magnitude of this phenomenon, we have developed an antigen receptor that is both optically and chemically tunable, providing control over the initiation, duration, and intensity of intracellular T-cell signaling within physiological cell conjugates. We observe very limited persistence within the T cell intracellular network on disruption of receptor input, with signals dissipating entirely in ~15 minutes, and directly confirm that sustained proximal receptor signaling is required to maintain active gene transcription. Our data suggests that T cells are largely incapable of integrating discrete antigen receptor signals but instead simply accumulate the output of gene expression. By engineering optical control in a clinically relevant chimeric antigen receptor, we show that this limited signal persistence can be exploited to increase the activation of primary T cells by ~3-fold by using pulsatile stimulation. Our results are likely to apply more generally to the signaling dynamics of other cellular networks.Competing Interest StatementThe authors have declared no competing interest.