PT  - JOURNAL ARTICLE
AU  - Michael K. Yu
AU  - Emily C. Fogarty
AU  - A. Murat Eren
TI  - Discovering plasmids in metagenomes based on genetic architecture
AID  - 10.1101/2020.11.01.361691
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.11.01.361691
4099  - http://biorxiv.org/content/early/2020/11/01/2020.11.01.361691.short
4100  - http://biorxiv.org/content/early/2020/11/01/2020.11.01.361691.full
AB  - Plasmids play a critical role in rapid bacterial adaptation by encoding accessory functions that may increase the host’s fitness. However, the diversity and ecology of plasmids is poorly understood due to computational and experimental challenges in plasmid identification. Here, we report the Plasmid Classification System (PCS), a machine learning classifier that recognizes plasmid sequences based on gene functions. To train PCS, we performed a large-scale discovery and comparison of gene functions in a reference set of >16,000 plasmids and >14,000 chromosomes. PCS accurately recognizes a diverse range of plasmid subtypes, and it outperforms the previous state-of-the-art approach based on k-mer decomposition of sequences. Armed with this model, we conducted, to our knowledge, the largest search for naturally occurring human gut plasmids in 406 publicly available metagenomes representing 5 countries. This search yielded 6,257 high-confidence predicted plasmids, of which 576 had evidence of a circular conformation based on pair-end mapping. These predicted plasmids were found to be highly prevalent across the metagenomes compared to the reference set of known plasmids, suggesting there is extensive and uncharacterized plasmid diversity in the human gut microbiome.Competing Interest StatementThe authors have declared no competing interest.