TY - JOUR T1 - Deep-learning based three-dimensional label-free tracking and analysis of immunological synapses of chimeric antigen receptor T cells JF - bioRxiv DO - 10.1101/539858 SP - 539858 AU - Moosung Lee AU - Young-Ho Lee AU - Jinyeop Song AU - Geon Kim AU - YoungJu Jo AU - HyunSeok Min AU - Chan Hyuk Kim AU - YongKeun Park Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/02/04/539858.abstract N2 - We propose and experimentally validate a label-free, volumetric, and automated assessment method of immunological synapse dynamics using a combinational approach of optical diffraction tomography and deep learning-based segmentation. The proposed approach enables automatic and quantitative spatiotemporal analyses of immunological synapse kinetics regarding morphological and biochemical parameters related to the total protein densities of immune cells, thus providing a new perspective for studies in immunology. ER -