PT  - JOURNAL ARTICLE
AU  - Larsson, Anton J. M.
AU  - Coucoravas, Christos
AU  - Sandberg, Rickard
AU  - Reinius, Björn
TI  - Transcriptional kinetics of X-chromosome upregulation
AID  - 10.1101/540104
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 540104
4099  - http://biorxiv.org/content/early/2019/02/05/540104.short
4100  - http://biorxiv.org/content/early/2019/02/05/540104.full
AB  - Ohno’s hypothesis postulates that X-chromosome upregulation rectifies X-dose imbalance relative to autosomal genes, present in two active copies per cell. Here we dissected X-upregulation into kinetics of transcription, inferred from allele-specific single-cell RNA-sequencing (scRNAseq) data from somatic mouse cells. We confirmed increased X-chromosome expression, and remarkably found that the X-chromosome achieved upregulation by elevated burst frequencies. This provides mechanistic insights into X-chromosome upregulation.