TY - JOUR T1 - Transcriptional Network Orchestrating Regional Patterning of Cortical Progenitors JF - bioRxiv DO - 10.1101/2020.11.03.366914 SP - 2020.11.03.366914 AU - Athéna R Ypsilanti AU - Kartik Pattabiraman AU - Rinaldo Catta-Preta AU - Olga Golonzhka AU - Susan Lindtner AU - Ke Tang AU - Ian Jones AU - Armen Abnousi AU - Ivan Juric AU - Ming Hu AU - Yin Shen AU - Diane E Dickel AU - Axel Visel AU - Len A Pennachio AU - Michael Hawrylycz AU - Carol Thompson AU - Hongkui Zeng AU - Iros Barozzi AU - Alex S Nord AU - John Rubenstein Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/11/04/2020.11.03.366914.abstract N2 - We uncovered a transcription factor (TF) network that regulates cortical regional patterning. Screening the expression of hundreds of TFs in the developing mouse cortex identified 38 TFs that are expressed in gradients in the ventricular zone (VZ). We tested whether their cortical expression was altered in mutant mice with known patterning defects (Emx2, Nr2f1 and Pax6), which enabled us to define a cortical regionalization TF network (CRTFN). To identify genomic programming underlying this network, we performed TF ChIP-seq and chromatin-looping conformation to identify enhancer-gene interactions. To map enhancers involved in regional patterning of cortical progenitors, we performed assays for epigenomic marks and DNA accessibility in VZ cells purified from wild-type and patterning mutant mice. This integrated approach has identified a CRTFN and VZ enhancers involved in cortical regional patterning.Competing Interest StatementThe authors have declared no competing interest. ER -