RT Journal Article
SR Electronic
T1 Spatial and temporal profiling of receptor membrane insertion controls commissural axon responses to midline repellents
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 540690
DO 10.1101/540690
A1 Aurora Pignata
A1 Hugo Ducuing
A1 Leila Boubakar
A1 Thibault Gardette
A1 Karine Kindbeiter
A1 Muriel Bozon
A1 Servane Tauszig-Delamasure
A1 Julien Falk
A1 Olivier Thoumine
A1 Valérie Castellani
YR 2019
UL http://biorxiv.org/content/early/2019/02/05/540690.abstract
AB Accurate perception of guidance cues is crucial for axonal pathfinding. During their initial navigation in the spinal cord, commissural axons are kept insensitive to midline repellents. Through yet unclear mechanisms acting during midline crossing in the floor plate, they switch on responsiveness to various repulsive signals, that establish a permanent midline barrier and propel the axons for exit. Whether these gains of response are coupled to occur in synchrony or rather are independently activated through signaling-specific programs is fully unknown. We set-up a paradigm for live imaging and super resolution analysis of guidance receptor dynamics during commissural growth cone navigation in chick and mouse embryos. We uncovered a remarkable program of delivery and allocation of receptors at the growth cone surface, generating receptor-specific spatial and temporal profiles. This reveals a mechanism whereby commissural growth cones can discriminate coincident repulsive signals that they functionalize at different time points of their navigation.