RT Journal Article
SR Electronic
T1 The host exocyst complex is targeted by a conserved bacterial type III effector protein that promotes virulence
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.11.06.371260
DO 10.1101/2020.11.06.371260
A1 Vassiliki A. Michalopoulou
A1 Konstantinos Kotsaridis
A1 Glykeria Mermigka
A1 Dina Kotsifaki
A1 Michael Kokkinidis
A1 Patrick H. N. Celie
A1 Jonathan D.G. Jones
A1 Panagiotis F. Sarris
YR 2020
UL http://biorxiv.org/content/early/2020/11/07/2020.11.06.371260.abstract
AB For most Gram-negative bacteria, pathogenicity largely depends on the type-III secretion system that delivers virulence effectors into eukaryotic cells. The subcellular targets for the majority of these effectors remain unknown. Here, we show that Xanthomonas campestris, the causal agent of black rot disease, delivers the highly conserved effector XopP that interacts with host’s EXO70 protein. EXO70 is an essential component of the exocyst complex with a role in plant immunity. The XopP/EXO70 interaction is specific and inhibits exocyst-dependent exocytosis without activating a specific plant NLR receptor that guards EXO70. In this way, Xanthomonas efficiently inhibits the host’s PAMP-triggered immunity (PTI) by blocking exocytosis of PR1, callose deposition and the FLS2 immunity-receptor translocation to the plasma membrane, promoting successful infection.Competing Interest StatementThe authors have declared no competing interest.