RT Journal Article
SR Electronic
T1 The DEAD-box RNA helicase Ded1 from yeast is associated with the signal recognition particle (SRP), and its enzymatic activity is regulated by SRP21
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.11.08.373522
DO 10.1101/2020.11.08.373522
A1 Hilal Yeter-Alat
A1 Naïma Belgareh-Touzé
A1 Emmeline Huvelle
A1 Molka Mokdadi
A1 Josette Banroques
A1 N. Kyle Tanner
YR 2020
UL http://biorxiv.org/content/early/2020/11/09/2020.11.08.373522.abstract
AB The DEAD-box RNA helicase Ded1 is an essential yeast protein involved in translation initiation. It belongs to the DDX3 subfamily of proteins implicated in developmental and cell-cycle regulation. In vitro, the purified Ded1 protein is an ATP-dependent RNA binding protein and an RNA-dependent ATPase, but it lacks RNA substrate specificity and enzymatic regulation. Here we demonstrate by yeast genetics, in situ localization and in vitro biochemical approaches that Ded1 is associated with, and regulated by, the signal recognition particle (SRP), which is a universally conserved ribonucleoprotein complex required for the co-translational translocation of polypeptides into the endoplasmic reticulum lumen and membrane. Ded1 is physically associated with SRP components in vivo and in vitro. Ded1 is genetically linked with SRP proteins. Finally, the enzymatic activity of Ded1 is inhibited by SRP21 with SCR1 RNA. We propose a model where Ded1 actively participates in the translocation of proteins during translation. Our results open a new comprehension of the cellular role of Ded1 during translation.