RT Journal Article
SR Electronic
T1 3D single cell scale anatomical map of sex-dependent variability of the rat intrinsic cardiac nervous system
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.07.29.227538
DO 10.1101/2020.07.29.227538
A1 Clara Leung
A1 Shaina Robbins
A1 Alison Moss
A1 Maci Heal
A1 Mahyar Osanlouy
A1 Richard Christie
A1 Navid Farahani
A1 Corey Monteith
A1 Jin Chen
A1 Peter Hunter
A1 Susan Tappan
A1 Rajanikanth Vadigepalli
A1 Zixi (Jack) Cheng
A1 James S. Schwaber
YR 2020
UL http://biorxiv.org/content/early/2020/11/10/2020.07.29.227538.abstract
AB We developed and analyzed a single cell scale anatomical map of the rat intrinsic cardiac nervous system (ICNS) across 4 male and 3 female hearts. We find the ICNS has a reliable structural organizational plan across individuals that may provide the foundation for further analyses of the ICNS in cardiac function and disease. The distribution of the ICNS was evaluated by 3D visualization and using data-driven clustering. The pattern, distribution and clustering of ICNS neurons across all male and female rat hearts is highly conserved, demonstrating a coherent organizational plan in which distinct clusters of neurons are consistently localized. Female hearts had fewer neurons, lower packing density and slightly reduced distribution, but with the same localization. We registered the anatomical data from each heart to a geometric scaffold, normalizing their 3D coordinates for the standardization of common anatomical planes and providing the path where multiple experimental results and data types can be integrated and compared.Competing Interest StatementM.H. and S.T. are paid employees at MBF Bioscience and are funded under the OT3OD025349, to create multi-scale, multi-organ, and multi-species SPARC map management as a part of the SPARC Data Portal. Strateos is a commercial entity, and the authors affiliated with them are company employees. The remaining authors declare no competing interests.