PT  - JOURNAL ARTICLE
AU  - Jiyeon Kweon
AU  - Jung-Ki Yoon
AU  - An-Hee Jang
AU  - Ha Rim Shin
AU  - Ji-Eun See
AU  - Gayoung Jang
AU  - Jong-Il Kim
AU  - Yongsub Kim
TI  - Engineered Prime Editors with PAM flexibility
AID  - 10.1101/2020.11.10.377580
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.11.10.377580
4099  - http://biorxiv.org/content/early/2020/11/11/2020.11.10.377580.short
4100  - http://biorxiv.org/content/early/2020/11/11/2020.11.10.377580.full
AB  - Although prime editors are a powerful tool for genome editing, which can generate various types of mutations such as nucleotide substitutions, insertions, and deletions in the genome without double-strand breaks or donor DNA, the conventional prime editors are still limited to its target scopes because of the PAM preference of the spCas9 protein. Here, we described the engineered prime editors to expand the range of their target sites using various PAM-flexible Cas9 variants.Competing Interest StatementThe authors have declared no competing interest.