RT Journal Article
SR Electronic
T1 Apoptotic Cells May Drive Cell Death in Hair Follicles During their Regression Cycle
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.11.16.384347
DO 10.1101/2020.11.16.384347
A1 Bradley D. Keister
A1 Kailin R. Mesa
A1 Krastan B. Blagoev
YR 2020
UL http://biorxiv.org/content/early/2020/11/16/2020.11.16.384347.abstract
AB Intravital microscopy in live mice have shown that the elimination of epithelial cells during hair follicle regression involves supra-basal cell differentiation and basal cell apoptosis through synergistic action of TGF-β (transforming growth factor) and mesenchymal-epithelial interactions. In this process the basal epithelial cells are not internally committed to death and the mesenchymal dermal papilla (DP) plays essential role in death induction. Because the DP cells are not necessary for completion of the cycle but only for its initiation it is still an open question what is the mechanism leading to the propagation of apoptosis towards the “immortal” stem cell population. Here, we use a quantitative analysis of the length of hair follicles during their regression cycle. The data is consistent with a propagation mechanism driven by apoptotic cells inducing apoptosis in their neighboring cells. The observation that the apoptosis slows down as the apoptotic front approaches the stem cells at the end of the follicle is consistent with a gradient of a pro-survival signal sent by these stem cells. An experiment that can falsify this mechanism is proposed.