RT Journal Article
SR Electronic
T1 Spatiotemporal dissection of the cell cycle regulated human proteome
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 543231
DO 10.1101/543231
A1 Diana Mahdessian
A1 Devin Sullivan
A1 Frida Danielsson
A1 Muhammad Arif
A1 Cheng Zhang
A1 Lovisa Åkesson
A1 Christian Gnann
A1 Rutger Shutten
A1 Peter Thul
A1 Oana Carja
A1 Burcu Ayoglu
A1 Adil Mardinoglu
A1 Fredrik Pontén
A1 Mathias Uhlén
A1 Cecilia Lindskog
A1 Emma Lundberg
YR 2019
UL http://biorxiv.org/content/early/2019/02/07/543231.abstract
AB Here we present a spatiotemporal dissection of proteome single cell heterogeneity in human cells, performed with subcellular resolution over the course of a cell cycle. We identify 17% of the human proteome to display cell-to-cell variability, of which we could attribute 25% as correlated to cell cycle progression, and present the first evidence of cell cycle association for 258 proteins. A key finding is that the variance, of many of the cell cycle associated proteins, is only partially explained by the cell cycle, which hints at cross-talk between the cell cycle and other signaling pathways. We also demonstrate that several of the identified cell cycle regulated proteins may be clinically significant in proliferative disorders. This spatially resolved proteome map of the cell cycle, integrated into the Human Protein Atlas, serves as a valuable resource to accelerate the molecular knowledge of the cell cycle and opens up novel avenues for the understanding of cell proliferation.