PT - JOURNAL ARTICLE AU - Hibbard, Jaime V.K. AU - Vazquez, Neftali AU - Satija, Rohit AU - Wallingford, John B. TI - Protein turnover dynamics suggest a diffusion to capture mechanism for peri-basal body recruitment and retention of intraflagellar transport proteins AID - 10.1101/2020.11.16.385518 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.11.16.385518 4099 - http://biorxiv.org/content/early/2020/11/17/2020.11.16.385518.short 4100 - http://biorxiv.org/content/early/2020/11/17/2020.11.16.385518.full AB - Intraflagellar transport (IFT) is essential for construction and maintenance of cilia. IFT proteins concentrate at the basal body, where they are thought to assemble into trains and bind cargoes for transport. To study the mechanisms of IFT recruitment to this peri-basal body pool, we quantified protein dynamics of eight IFT proteins, as well as five other basal body localizing proteins, using fluorescence recovery after photobleaching in vertebrate multiciliated cells. We found that members of the IFT-A and IFT-B protein complexes show distinct turnover kinetics from other basal body components. Additionally, known IFT sub-complexes displayed shared dynamics, and these dynamics were not altered during cilia regeneration as compared to homeostasis. Finally, we evaluated the mechanisms of basal body recruitment by depolymerizing cytosolic MTs, which suggested that IFT proteins are recruited to basal bodies through a diffusion-to-capture mechanism. Our survey of IFT protein dynamics provides new insights into IFT recruitment to basal bodies, a crucial step in ciliogenesis.Competing Interest StatementThe authors have declared no competing interest.