RT Journal Article
SR Electronic
T1 Sub-centrosomal mapping identifies augmin-γTuRC as part of a centriole-stabilizing scaffold
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.11.18.384156
DO 10.1101/2020.11.18.384156
A1 Nina Schweizer
A1 Laurence Haren
A1 Ricardo Viais
A1 Cristina Lacasa
A1 Ilaria Dutto
A1 Andreas Merdes
A1 Jens Lüders
YR 2020
UL http://biorxiv.org/content/early/2020/11/18/2020.11.18.384156.abstract
AB Centriole biogenesis and maintenance are crucial for cells to generate cilia and assemble centrosomes that function as microtubule organizing centers (MTOCs). Centriole biogenesis and MTOC function both require the microtubule nucleator γ-tubulin ring complex (γTuRC). The widely accepted view is that γTuRC localizes to the pericentriolar material (PCM), where it nucleates microtubules. γTuRC has also been observed at centriolar regions that lack PCM, but the significance of these findings is unclear. Here we have used expansion microscopy to map spatially and functionally distinct sub-populations of centrosomal γTuRC including in the centriole lumen. Luminal localization is mediated by augmin and both complexes are linked to the centriole inner scaffold through POC5. Disruption of luminal localization impairs centriole stability and cilia assembly, defects that are also observed in γTuRC mutant fibroblasts derived from a patient suffering from microcephaly with chorioretinopathy. These results identify a novel, non-canonical role of augmin-γTuRC in the centriole lumen that is linked to human disease.Competing Interest StatementThe authors have declared no competing interest.