RT Journal Article
SR Electronic
T1 The centriolar satellite protein Cfap53/Ccdc11 facilitates the formation of the first zygotic microtubule organizing center in the zebrafish embryo
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.11.18.388652
DO 10.1101/2020.11.18.388652
A1 Sven Willekers
A1 Federico Tessadori
A1 Babet van der Vaart
A1 Heiko Henning
A1 Riccardo Stucchi
A1 Maarten Altelaar
A1 Bernard A.J. Roelen
A1 Anna Akhmanova
A1 Jeroen Bakkers
YR 2020
UL http://biorxiv.org/content/early/2020/11/18/2020.11.18.388652.abstract
AB In embryos from most animal species a zygotic centrosome is assembled by the centriole derived from the sperm cell and pericentriolar proteins present in the oocyte. This zygotic centrosome acts as a microtubule organizing center (MTOC) to assemble the mitotic spindle in the first and all subsequent cell divisions. As MTOC formation has been studied mainly in adult cells, very little is known about the formation of the first zygotic MTOC. Here we find that zebrafish (Danio rerio) embryos lacking maternal or paternal Cfap53, a centriolar satellite protein, arrest during the first cell cycle due to a failure in proper formation of the mitotic spindle. During the first cell cycle Cfap53 co-localizes with γ-tubulin and other centrosomal and centriolar satellite proteins to the very large MTOC. Furthermore, we find that γ-tubulin localization to the MTOC is impaired in the absence of Cfap53 or when the microtubule network is disrupted. Based on these results we propose a model in which maternal and paternal Cfap53 participates in the organization of the first zygotic MTOC of the embryo. Once the zygotic MTOC is formed, Cfap53 is dispensable for MTOC formation and integrity in subsequent cell divisions.