TY - JOUR T1 - Dynamics of gene expression and chromatin marking during cell state transition JF - bioRxiv DO - 10.1101/2020.11.20.391524 SP - 2020.11.20.391524 AU - Beatrice Borsari AU - Amaya Abad AU - Cecilia C. Klein AU - Ramil Nurtdinov AU - Alexandre Esteban AU - Emilio Palumbo AU - Marina Ruiz-Romero AU - María Sanz AU - Bruna R. Correa AU - Rory Johnson AU - Sílvia Pérez-Lluch AU - Roderic Guigó Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/11/20/2020.11.20.391524.abstract N2 - We have monitored the transcriptomic and epigenomic status of cells at twelve time-points during the transdifferentiation of human pre-B cells into macrophages. Using this data, we have investigated some fundamental questions regarding the role of chromatin in gene expression. We have found that, over time, genes are characterized by a limited number of chromatin states (combinations of histone modifications), and that, consistently, chromatin changes over genes tend to occur in a coordinated manner. We have observed strong association between these changes and gene expression only at the time of initial gene activation. Activation is preceded by H3K4me1 and H3K4me2, and followed in a precise order by most other histone modifications. Further changes in gene expression, comparable or even stronger than those at initial activation, occur without associated changes in histone modifications. The data generated here constitutes, thus, a unique resource to investigate transcriptomic and epigenomic dynamics during a differentiation process.Competing Interest StatementThe authors have declared no competing interest. ER -