PT  - JOURNAL ARTICLE
AU  - Huascar Pedro Ortuste Quiroga
AU  - Shingo Yokoyama
AU  - Massimo Ganassi
AU  - Kodai Nakamura
AU  - Tomohiro Yamashita
AU  - Daniel Raimbach
AU  - Arisa Hagiwara
AU  - Atsushi Asakura
AU  - Yoshiro Suzuki
AU  - Makoto Tominaga
AU  - Peter S. Zammit
AU  - Katsumasa Goto
TI  - Mechanosensitive Ion Channel Piezo1 Regulates Myocyte Fusion during Skeletal Myogenesis
AID  - 10.1101/2020.09.27.315242
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.09.27.315242
4099  - http://biorxiv.org/content/early/2020/11/22/2020.09.27.315242.short
4100  - http://biorxiv.org/content/early/2020/11/22/2020.09.27.315242.full
AB  - Mechanical stimuli such as stretch and resistance training are essential to regulate growth and function of skeletal muscle. However, the molecular mechanisms involved in sensing mechanical stress remain unclear. Here, the purpose of this study was to investigate the role of the mechanosensitive ion channel Piezo1 during myogenic progression. Muscle satellite cell-derived myoblasts and myotubes were modified with stretch, siRNA knockdown and agonist-induced activation of Piezo1. Direct manipulation of Piezo1 modulates terminal myogenic progression. Piezo1 knockdown suppressed myoblast fusion during myotube formation and maturation. This was accompanied by downregulation of the fusogenic protein Myomaker. Piezo1 knockdown also lowered Ca2+ influx in response to stretch. Conversely Piezo1 activation stimulated fusion and increased Ca2+ influx in response to stretch. These evidences indicate that Piezo1 is essential for myotube formation and maturation, which may have implications for msucular dystrophy prevention through its role as a mechanosensitive Ca2+ channel.Competing Interest StatementThe authors have declared no competing interest.