PT  - JOURNAL ARTICLE
AU  - Neville E. Sanjana
AU  - Ophir Shalem
AU  - Feng Zhang
TI  - Improved vectors and genome-wide libraries for CRISPR screening
AID  - 10.1101/006726
DP  - 2014 Jan 01
TA  - bioRxiv
PG  - 006726
4099  - http://biorxiv.org/content/early/2014/06/28/006726.short
4100  - http://biorxiv.org/content/early/2014/06/28/006726.full
AB  - Genome-wide, targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)–associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi) and have been used to reveal new mechanisms in diverse biological models1–4. Previously, we used a Genome-scale CRISPR Knock-Out (GeCKO) library to identify loss-of-function mutations conferring vemurafenib resistance in a melanoma model1. However, initial lentiviral delivery systems for CRISPR screening had low viral titer or required a cell line already expressing Cas9, limiting the range of biological systems amenable to screening.