RT Journal Article
SR Electronic
T1 Improved vectors and genome-wide libraries for CRISPR screening
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 006726
DO 10.1101/006726
A1 Sanjana, Neville E.
A1 Shalem, Ophir
A1 Zhang, Feng
YR 2014
UL http://biorxiv.org/content/early/2014/06/28/006726.abstract
AB Genome-wide, targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)–associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi) and have been used to reveal new mechanisms in diverse biological models1–4. Previously, we used a Genome-scale CRISPR Knock-Out (GeCKO) library to identify loss-of-function mutations conferring vemurafenib resistance in a melanoma model1. However, initial lentiviral delivery systems for CRISPR screening had low viral titer or required a cell line already expressing Cas9, limiting the range of biological systems amenable to screening.