RT Journal Article SR Electronic T1 Improved vectors and genome-wide libraries for CRISPR screening JF bioRxiv FD Cold Spring Harbor Laboratory SP 006726 DO 10.1101/006726 A1 Sanjana, Neville E. A1 Shalem, Ophir A1 Zhang, Feng YR 2014 UL http://biorxiv.org/content/early/2014/06/28/006726.abstract AB Genome-wide, targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)–associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi) and have been used to reveal new mechanisms in diverse biological models1–4. Previously, we used a Genome-scale CRISPR Knock-Out (GeCKO) library to identify loss-of-function mutations conferring vemurafenib resistance in a melanoma model1. However, initial lentiviral delivery systems for CRISPR screening had low viral titer or required a cell line already expressing Cas9, limiting the range of biological systems amenable to screening.