RT Journal Article
SR Electronic
T1 Improved vectors and genome-wide libraries for CRISPR screening
JF bioRxiv
FD Cold Spring Harbor Laboratory Press
DO 10.1101/006726
A1 Sanjana, Neville E
A1 Shalem, Ophir
A1 Zhang, Feng
YR 2014
UL http://biorxiv.org/content/early/2014/06/28/006726.abstract
AB Genome-wide, targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)?associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi). Initial lentiviral delivery systems for CRISPR screening had low viral titer or required a cell line already expressing Cas9, limiting the range of biological systems amenable to screening. In this work, we present 1- and 2-vector lentiCRISPR systems capable of producing higher viral titers and, in these vectors, new human and mouse libraries for genome-scale CRISPR knock-out (GeCKO) screening.