RT Journal Article
SR Electronic
T1 Multi-parameter formulation development for an HIV-vaccine protein with direct validation of epitope binding integrity and stoichiometry
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.11.30.403873
DO 10.1101/2020.11.30.403873
A1 Corinna Popp
A1 Philipp Schramm
A1 Ralf Wagner
A1 David Peterhoff
A1 Christopher Battle
A1 Christian Kleusch
A1 Maximilian G. Plach
YR 2020
UL http://biorxiv.org/content/early/2020/11/30/2020.11.30.403873.abstract
AB Vaccines are on the front line-of-defense against infectious diseases, ranging from threats we are familiar with, including polio, tuberculosis, or HIV, to novel and emerging threats such as SARS-CoV-2. Successful development of new protein-based vaccines requires sophisticated and efficient development of storage and formulation conditions. Here we demonstrate the combined power of 2bind’s sophisticated buffer matrix FORMOscreen® and NanoTemper Technologies’ novel Prometheus Panta high-throughput Dynamic Light Scattering/Nano Differential Scanning Fluorimetry instrument. We show that this combination can comprehensively improve critical biophysical parameters for the HIV-1 vaccine BG505-SOSIP and find the optimal formulation condition with unmatched efficiency.Competing Interest StatementThe authors have declared no competing interest.