RT Journal Article
SR Electronic
T1 Transcriptional profiling reveals potential involvement of microvillous TRPM5-expressing cells in viral infection of the olfactory epithelium
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.14.096016
DO 10.1101/2020.05.14.096016
A1 B. Dnate’ Baxter
A1 Eric D. Larson
A1 Laetitia Merle
A1 Paul Feinstein
A1 Arianna Gentile Polese
A1 Andrew N. Bubak
A1 Christy S. Niemeyer
A1 James Hassell, Jr.
A1 Doug Shepherd
A1 Vijay R. Ramakrishnan
A1 Maria A. Nagel
A1 Diego Restrepo
YR 2020
UL http://biorxiv.org/content/early/2020/12/01/2020.05.14.096016.abstract
AB Background Understanding viral infection of the olfactory epithelium is essential because the olfactory nerve is an important route of entry for viruses to the central nervous system. Specialized chemosensory epithelial cells that express the transient receptor potential cation channel subfamily M member 5 (TRPM5) are found throughout the airways and intestinal epithelium and are involved in responses to viral infection.Results Herein we performed deep transcriptional profiling of olfactory epithelial cells sorted by flow cytometry based on the expression of mCherry as a marker for olfactory sensory neurons and for eGFP in OMP-H2B::mCherry/TRPM5-eGFP transgenic mice (Mus musculus). We find profuse expression of transcripts involved in inflammation, immunity and viral infection in TRPM5-expressing microvillous cells.Conclusion Our study provides new insights into a potential role for TRPM5-expressing microvillous cells in viral infection of the olfactory epithelium. We find that, as found for solitary chemosensory cells (SCCs) and brush cells in the airway epithelium, and for tuft cells in the intestine, the transcriptome of TRPM5-expressing microvillous cells indicates that they are likely involved in the inflammatory response elicited by viral infection of the olfactory epithelium.Competing Interest StatementThe authors have declared no competing interest.