TY - JOUR T1 - Mechanisms of antibody binding revealed by asymmetric Fab-virus complexes JF - bioRxiv DO - 10.1101/2020.12.01.406983 SP - 2020.12.01.406983 AU - Daniel J. Goetschius AU - Samantha R. Hartmann AU - Lindsey J. Organtini AU - Heather Callaway AU - Kai Huang AU - Carol M. Bator AU - Robert E. Ashley AU - Alexander M. Makhov AU - James F. Conway AU - Colin R. Parrish AU - Susan Hafenstein Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/12/01/2020.12.01.406983.abstract N2 - Overlap on the surface of parvovirus capsids between the antigenic epitope and the receptor binding site contributes to species jumping. Mab 14 strongly binds and neutralizes canine, but not feline parvovirus. The high resolution map of the canine parvovirus capsid complexed with Fab 14 was used to solve local structures of the Fab-bound and -unbound antigenic sites extracted from the same complex. The subsequent analysis includes a new method for using cryo EM to investigate complementarity of antibody binding.Competing Interest StatementThe authors have declared no competing interest. ER -