RT Journal Article
SR Electronic
T1 Touch DNA on objects can be analysed at low cost using simplified direct amplification methods
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 540823
DO 10.1101/540823
A1 Katherine Gammon
A1 Kirk Murray-Jones
A1 Daniel Shenton
A1 Zoe Wood
A1 Carl Mayers
YR 2019
UL http://biorxiv.org/content/early/2019/02/08/540823.abstract
AB Previous studies reported in the literature demonstrate that a range of sampling vehicles can be used effectively for forensic analysis of human DNA in direct amplification reactions. In this study we compared Copan microFLOQ® swabs with a range of alternative sampling vehicles, using touch DNA samples donated by 15 different volunteers. MicroFLOQ swabs performed well, as did 3 mm diameter discs punched from analytical filter paper. The 3 mm discs could be used in a 5 µl PCR volume, increasing sensitivity, and reducing costs when compared with other methods that require a larger PCR volume. Other inert sampling vehicles, such as interdental toothbrushes and toothpicks also gave good results in direct amplification. The study found a large variation in results between the 15 touch DNA donors, demonstrating the importance of validating touch DNA recovery techniques with a large pool of donors.