TY - JOUR T1 - Single-cell intracellular epitope and transcript detection revealing signal transduction dynamics JF - bioRxiv DO - 10.1101/2020.12.02.408120 SP - 2020.12.02.408120 AU - Francesca Rivello AU - Erik van Buijtenen AU - Kinga MatuĊ‚a AU - Jessie A.G.L. van Buggenum AU - Paul Vink AU - Hans van Eenennaam AU - Klaas W. Mulder AU - Wilhelm T. S. Huck Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/12/02/2020.12.02.408120.abstract N2 - Current high-throughput single-cell multi-omics methods cannot concurrently map changes in (phospho)protein levels and the associated gene expression profiles. We present QuRIE-seq (Quantification of RNA and Intracellular Epitopes by sequencing) and use multi-factor omics analysis (MOFA+) to map signal transduction over multiple timescales. We demonstrate that QuRIE-seq can trace the activation of the B-cell receptor pathway at the minute and hour time-scale and provide insight into the mechanism of action of an inhibitory drug, Ibrutinib.Competing Interest StatementThe authors have declared no competing interest. ER -