RT Journal Article
SR Electronic
T1 Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.15.095174
DO 10.1101/2020.05.15.095174
A1 Frøydis Sved Skottvoll
A1 Frederik Hansen
A1 Sean Harrison
A1 Ida Sneis Boger
A1 Ago Mrsa
A1 Magnus Saed Restan
A1 Matthias Stein
A1 Elsa Lundanes
A1 Stig Pedersen-Bjergaard
A1 Aleksandra Aizenshtadt
A1 Stefan Krauss
A1 Gareth Sullivan
A1 Inger Lise Bogen
A1 Steven Ray Wilson
YR 2020
UL http://biorxiv.org/content/early/2020/12/04/2020.05.15.095174.abstract
AB Liver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry (MS)-based measurements. EME, allowed drugs and drug metabolites to be separated from cell medium components (albumin, etc.) that could interfere with subsequent measurements. Multi-well EME (Parallel-EME) holding 100 μL solutions allowed for simple and repeatable monitoring of heroin phase I metabolism kinetics. Organoid Parallel-EME extracts were compatible with ultrahigh-performance liquid chromatography (UHPLC) used to separate the analytes prior to detection. Taken together, liver organoids are well-matched with EME followed by MS-based measurements.Competing Interest StatementThe authors have declared no competing interest.