PT  - JOURNAL ARTICLE
AU  - Sareh Arjmand
AU  - Nazanin Hosseinkhan
TI  - Identification of potential coagulation pathway abnormalities in SARS-Cov-2 infection; insights from bioinformatics analysis
AID  - 10.1101/2020.12.07.414631
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.12.07.414631
4099  - http://biorxiv.org/content/early/2020/12/07/2020.12.07.414631.short
4100  - http://biorxiv.org/content/early/2020/12/07/2020.12.07.414631.full
AB  - Abnormal coagulation parameters have been explored in a significant number of severe COVID-19 patients, linked to poor prognosis and increased risk of organ failure. Here, to uncover the potential abnormalities in coagulation pathways, we analyzed the RNA-seq data (GEO147507) obtained from the treatment of three pulmonary epithelial cell lines with SARS-CoV-2. The significant differentially expressed genes (DEGs) were subjected to Enrichr database for KEGG pathway enrichment analysis and gene ontology (GO) functional annotation. The STRING database was used to generate PPI networks for identified DEGs. We found three upregulated procoagulant genes (SERPINE1, SERPINA5, and SERPINB2) belong to the serine protease inhibitor (serpin) superfamily that inhibit tissue plasminogen activator (t-PA) and urokinase plasminogen activator (u-PA) in the fibrinolysis process. In conclusion, we suggest the fibrinolysis process, especially the blockage of t-PA and u-PA inhibitors, a potential target for more study in treating coagulopathy in severe COVID-19 cases.Competing Interest StatementThe authors have declared no competing interest.