RT Journal Article
SR Electronic
T1 Multiple clones of colistin-resistant <em>Salmonella enterica</em> carrying <em>mcr-1</em> plasmids in meat products and patients in Northern Thailand
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.12.08.415869
DO 10.1101/2020.12.08.415869
A1 Patchanee, Prapas
A1 Chokesajjawatee, Nipa
A1 Santiyanont, Pannita
A1 Chuammitri, Phongsakorn
A1 Deeudom, Manu
A1 Monteith, William
A1 Sheppard, Samuel K.
A1 Pascoe, Ben
A1 Prasertsee, Teerarat
YR 2020
UL http://biorxiv.org/content/early/2020/12/08/2020.12.08.415869.abstract
AB Salmonella spp. is an important foodborne pathogen associated with consumption of contaminated food, especially livestock products. Antimicrobial resistance (AMR) in Salmonella has been reported globally and increasing AMR in food production is a major public health issue worldwide. The objective of this study was to describe the genetic relatedness among Salmonella enterica isolates, which displayed identical DNA fingerprint profiles. Ten S. enterica isolates were selected from meat and human cases with an identical rep-PCR profile of serovars Rissen (n=4), Weltevreden (n=4), and Stanley (n=2). We used long-read whole genome sequencing (WGS) on the MinION sequencing platform to type isolates and investigate in silico the presence of specific AMR genes. Antimicrobial susceptibility testing was tested by disk diffusion and gradient diffusion method to corroborate the AMR phenotype. Multidrug resistance and resistance to more than one antimicrobial agent were observed in eight and nine isolates, respectively. Resistance to colistin with an accompanying mcr-1 gene was observed among the Salmonella isolates. The analysis of core genome and whole genome MLST revealed that the Salmonella from meat and human salmonellosis were closely genetic related. Hence, it could be concluded that meat is one of the important sources for Salmonella infection in human.HighlightsColistin resistance detected in 2 clones from 2 different Salmonella enterica serovars (Rissen and Weltevreden) with accompanying plasmid-borne mcr-1 gene from the food production chain and human clinical salmonellosis.High prevalence of multidrug resistant isolates and resistance to more than one antimicrobial agent.MinION has potential for mobile, rapid and accurate application in veterinary genomic epidemiology studies.Competing Interest StatementThe authors have declared no competing interest.