TY - JOUR T1 - Impaired phosphatidylethanolamine metabolism activates a reversible stress response that detects and resolves mutant mitochondrial precursors JF - bioRxiv DO - 10.1101/2020.12.10.416495 SP - 2020.12.10.416495 AU - Pingdewinde N. Sam AU - Elizabeth Calzada AU - Michelle Grace Acoba AU - Tian Zhao AU - Yasunori Watanabe AU - Anahita Nejatfard AU - Jonathan C. Trinidad AU - Timothy E. Shutt AU - Sonya E. Neal AU - Steven M. Claypool Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/12/11/2020.12.10.416495.abstract N2 - Phosphatidylethanolamine made in mitochondria has long been recognized as an important precursor for phosphatidylcholine production that occurs in the endoplasmic reticulum (ER). Recently, the strict mitochondrial localization of the enzyme that makes PE in the mitochondrion, phosphatidylserine decarboxylase 1 (Psd1), was questioned. Since a dual localization of Psd1 to the ER would have far-reaching implications, we initiated our study to independently re-assess the subcellular distribution of Psd1. Our results support the unavoidable conclusion that the vast majority, if not all, of functional Psd1 resides in the mitochondrion. Through our efforts, we discovered that mutant forms of Psd1 that impair a self-processing step needed for it to become functional are dually localized to the ER when expressed in a PE-limiting environment. We conclude that severely impaired cellular PE metabolism provokes an ER-assisted adaptive response that is capable of identifying and resolving nonfunctional mitochondrial precursors.Competing Interest StatementThe authors have declared no competing interest. ER -