@article {Ando2020.03.30.015156, author = {Ryoko Ando and Asako Sakaue-Sawano and Keiko Shoda and Atsushi Miyawaki}, title = {Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression}, elocation-id = {2020.03.30.015156}, year = {2020}, doi = {10.1101/2020.03.30.015156}, publisher = {Cold Spring Harbor Laboratory}, abstract = {We cloned and characterized two new coral fluorescent proteins: h2-3 and 1-41. h2-3 formed an obligate dimeric complex and exhibited bright fluorescence. On the other hand, 1-41 formed a highly multimeric complex and exhibited dim red fluorescence. We engineered 1-41 into AzaleaB5, a practically useful red-emitting fluorescent protein for cellular labeling applications. We fused h2-3 and AzaleaB5 to the ubiquitination domains of human Geminin and Cdt1, respectively, to generate a new color variant of Fucci (Fluorescent Ubiquitination-based Cell-Cycle Indicator): Fucci5. We found Fucci5 provided brighter nuclear labeling for monitoring cell cycle progression than the 1st and 2nd generations that used mAG/mKO2 and mVenus/mCherry, respectively.Competing Interest StatementThe authors have declared no competing interest.}, URL = {https://www.biorxiv.org/content/early/2020/12/15/2020.03.30.015156}, eprint = {https://www.biorxiv.org/content/early/2020/12/15/2020.03.30.015156.full.pdf}, journal = {bioRxiv} }