PT - JOURNAL ARTICLE AU - Ryoko Ando AU - Asako Sakaue-Sawano AU - Keiko Shoda AU - Atsushi Miyawaki TI - Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression AID - 10.1101/2020.03.30.015156 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.03.30.015156 4099 - http://biorxiv.org/content/early/2020/12/15/2020.03.30.015156.short 4100 - http://biorxiv.org/content/early/2020/12/15/2020.03.30.015156.full AB - We cloned and characterized two new coral fluorescent proteins: h2-3 and 1-41. h2-3 formed an obligate dimeric complex and exhibited bright fluorescence. On the other hand, 1-41 formed a highly multimeric complex and exhibited dim red fluorescence. We engineered 1-41 into AzaleaB5, a practically useful red-emitting fluorescent protein for cellular labeling applications. We fused h2-3 and AzaleaB5 to the ubiquitination domains of human Geminin and Cdt1, respectively, to generate a new color variant of Fucci (Fluorescent Ubiquitination-based Cell-Cycle Indicator): Fucci5. We found Fucci5 provided brighter nuclear labeling for monitoring cell cycle progression than the 1st and 2nd generations that used mAG/mKO2 and mVenus/mCherry, respectively.Competing Interest StatementThe authors have declared no competing interest.