TY - JOUR T1 - Translation factor mRNA granules direct protein synthetic capacity to regions of polarized growth JF - bioRxiv DO - 10.1101/447680 SP - 447680 AU - Mariavittoria Pizzinga AU - Christian Bates AU - Jennifer Lui AU - Gabriella Forte AU - Fabián Morales-Polanco AU - Emma Linney AU - Barbora Knotkova AU - Beverley Wilson AU - Clara A. Solari AU - Luke E. Berchowitz AU - Paula Portela AU - Mark P. Ashe Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/02/09/447680.abstract N2 - mRNA localization serves key functions in localized protein production making it critical that the translation machinery itself is present at these locations. Here we show that translation factor mRNAs are localized to distinct granules within yeast cells. In contrast to many mRNP granules, such as P-bodies and stress granules, which contain translationally repressed mRNAs, these granules harbor translated mRNAs under active growth conditions. The granules require Pablp for their integrity and are inherited by developing daughter cells in a She2p/ She3p dependent manner. These results point to a model where roughly half the mRNA for certain translation factors are specifically directed in granules toward the tip of the developing daughter cell where protein synthesis is most heavily required, which has particular implications for filamentous forms of growth. Such a feedforward mechanism would ensure adequate provision of the translation machinery where it is to be needed most over the coming growth cycle.Summary This study shows that mRNAs encoding a range of translation factors are localized to granules that get transported into the yeast daughter cell using the She2p/She3p machinery. This likely supports an intensification of protein synthetic activity to facilitate apical polarized growth. ER -