RT Journal Article
SR Electronic
T1 Translation factor mRNA granules direct protein synthetic capacity to regions of polarized growth
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 447680
DO 10.1101/447680
A1 Mariavittoria Pizzinga
A1 Christian Bates
A1 Jennifer Lui
A1 Gabriella Forte
A1 Fabián Morales-Polanco
A1 Emma Linney
A1 Barbora Knotkova
A1 Beverley Wilson
A1 Clara A. Solari
A1 Luke E. Berchowitz
A1 Paula Portela
A1 Mark P. Ashe
YR 2019
UL http://biorxiv.org/content/early/2019/02/09/447680.abstract
AB mRNA localization serves key functions in localized protein production making it critical that the translation machinery itself is present at these locations. Here we show that translation factor mRNAs are localized to distinct granules within yeast cells. In contrast to many mRNP granules, such as P-bodies and stress granules, which contain translationally repressed mRNAs, these granules harbor translated mRNAs under active growth conditions. The granules require Pablp for their integrity and are inherited by developing daughter cells in a She2p/ She3p dependent manner. These results point to a model where roughly half the mRNA for certain translation factors are specifically directed in granules toward the tip of the developing daughter cell where protein synthesis is most heavily required, which has particular implications for filamentous forms of growth. Such a feedforward mechanism would ensure adequate provision of the translation machinery where it is to be needed most over the coming growth cycle.Summary This study shows that mRNAs encoding a range of translation factors are localized to granules that get transported into the yeast daughter cell using the She2p/She3p machinery. This likely supports an intensification of protein synthetic activity to facilitate apical polarized growth.