RT Journal Article
SR Electronic
T1 Detection of long SARS-CoV-2 nucleocapsid sequences in peripheral blood monocytes collected soon after hospital admission
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.12.16.423113
DO 10.1101/2020.12.16.423113
A1 Pagano, Nathan
A1 Laurent-Rolle, Maudry
A1 Chun-Chieh Hsu, Jack
A1 ,
A1 Vogels, Chantal BF
A1 Grubaugh, Nathan D
A1 Manuelidis, Laura
YR 2020
UL http://biorxiv.org/content/early/2020/12/16/2020.12.16.423113.abstract
AB Many different viruses infect circulating mononuclear cells to disseminate infection to diverse organs. Blood mononuclear cells (PBMC) are being intensively studied as immunologic and pathologic responders to the new pandemic SARS-CoV-2 virus (CoV19) but direct evidence showing CoV19 in these cells has not been published. PBMC myeloid cells that take up residence in various organs can harbor viral genomes for many years in lymphatic tissues, skin and brain, and act as a source for re-infection and/or post-viral organ pathology. To test if PBMC from acutely ill hospitalized patients contain viral nucleic acids, we first analyzed a standard short CoV19 nucleocapsid (NC) 72bp sequence. Because NC proteins protect the viral genome, we further analyzed longer (301nt) adjacent NC stretches by RNA/qPCR. In 2 of 11 patient PBMC, but no uninfected controls, longer NC sequences were positive as early as 2-6 days after hospital admission and were validated by sequencing. The presence of longer NC sequences indicates pathogenic fragments, or possibly the complete infectious virus, are carried by a rare population of monocytes, probably a subset of myeloid migratory cells. Predictably, such cells carried CoV19 to heart and brain with consequent late post-viral immune pathologies that are now evident.Competing Interest StatementThe authors have declared no competing interest.