RT Journal Article
SR Electronic
T1 Quantifying absolute gene expression profiles reveals distinct regulation of central carbon metabolism genes in yeast
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.12.22.423946
DO 10.1101/2020.12.22.423946
A1 Rosemary Yu
A1 Egor Vorontsov
A1 Carina Sihlbom
A1 Jens Nielsen
YR 2020
UL http://biorxiv.org/content/early/2020/12/22/2020.12.22.423946.abstract
AB In addition to specific regulatory circuits, gene expression is also regulated by global physiological cues such as the cell growth rate and metabolic parameters. Here we examine these global control mechanisms by analyzing an orthogonal multi-omics dataset consisting of absolute-quantitative abundances of the transcriptome, proteome, and intracellular amino acids in 22 steady-state yeast cultures. Our model indicates that transcript and protein abundance are coordinately controlled by the cell growth rate via RNA polymerase II and ribosome abundance, but are independently controlled by metabolic parameters relating to amino acid and nucleotide availability. Genes in central carbon metabolism, however, are regulated independently of these global physiological cues. Our findings can be used to augment gene expression profiling analyses in the distantly related yeast Schizosaccharomyces pombe and a human cancer cell model. Our results provide a framework to analyze gene expression profiles to gain novel biological insights, a key goal of systems biology.Competing Interest StatementJ.N. is the CEO of the BioInnovation Institute, Denmark.