PT  - JOURNAL ARTICLE
AU  - Luoto, Jens C.
AU  - Coelho-Rato, Leila S.
AU  - Bengs, Sara H.
AU  - Roininen, Jannica
AU  - Eriksson, John E.
AU  - Sistonen, Lea
AU  - Henriksson, Eva
TI  - <em>In vivo</em>-mimicking 3D cultures secrete distinct extracellular vesicles upon cancer cell invasion
AID  - 10.1101/2020.12.22.423913
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.12.22.423913
4099  - http://biorxiv.org/content/early/2020/12/23/2020.12.22.423913.short
4100  - http://biorxiv.org/content/early/2020/12/23/2020.12.22.423913.full
AB  - Extracellular vesicles (EVs) loaded with biomolecules are important in intercellular communication and mediate local and long-range signals in cancer metastasis. However, it is currently unknown how the development of the primary tumor and onset of invasion affect the secretion and characteristics of EVs. In this study, we developed an EV production method utilizing in vivo-mimicking extracellular matrix-based 3D cultures, which allows tracking of EVs over the course of invasive development of tumor organoids. Using this method, combined with proteomic profiling, we show that PC3 human prostate cancer organoids secrete EVs with previously undefined protein cargo, which substantially differs from EV cargo of 2D cultured cells. Intriguingly, an increase in EV amounts and extensive changes in EV protein composition were detected upon invasive transition of the organoids. These results reveal that EV secretion and cargo loading are highly dependent on the developmental status of the tumor organoid, emphasizing the necessity of in vivo-mimicking conditions for discovery of novel cancer-derived EV components, applicable as diagnostic markers for cancer.