PT - JOURNAL ARTICLE AU - Fernando A. Villanea AU - Emilia Huerta-Sanchez AU - P. Keolu Fox TI - ABO genetic variation in Neanderthals and Denisovans AID - 10.1101/2020.07.27.223628 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.07.27.223628 4099 - http://biorxiv.org/content/early/2020/12/24/2020.07.27.223628.short 4100 - http://biorxiv.org/content/early/2020/12/24/2020.07.27.223628.full AB - Variation at the ABO locus was one of the earliest sources of data in the study of human population identity and history, and to this day remains widely genotyped due to its importance in blood and tissue transfusions. Here, we look at ABO blood type variants in our archaic relatives: Neanderthals and Denisovans. Our goal is to understand the genetic landscape of the ABO gene in archaic humans, and how it relates to modern human ABO variation. We found two derived Neanderthal variants of the O allele in the Siberian Neanderthals (O1 and O2), one of these variants is shared with an European Neanderthal, who is a heterozygote for this O1 variant and a rare cis-AB variant. The Denisovan individual is heterozygous for two ancestral variant of the O1 allele, similar to variants found widely in modern humans. Perhaps more surprisingly, the O2 allele variant found in Siberian Neanderthals can be found at low frequencies in modern Europeans and Southeast Asians, and the O1 allele variant found in Siberian and European Neanderthal is also found at very low frequency in modern East Asians. Our genetic distance analyses suggest both alleles survive in modern humans due to inbreeding with Neanderthals. We find that the sequence backgrounds of the surviving Neanderthal-like O alleles in modern humans retain a higher sequence divergence than other surviving Neanderthal genome fragments, supporting a view of balancing selection operating in the Neanderthal ABO alleles by retaining highly diverse haplotypes compared to portions of the genome evolving neutrally.Competing Interest StatementThe authors have declared no competing interest.