TY - JOUR T1 - XMAP215 promotes microtubule catastrophe by disrupting the growing microtubule end JF - bioRxiv DO - 10.1101/2020.12.29.424748 SP - 2020.12.29.424748 AU - Veronica Farmer AU - Göker Arpağ AU - Sarah Hall AU - Marija Zanic Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/12/29/2020.12.29.424748.abstract N2 - The GTP-tubulin cap is widely accepted to protect microtubules against catastrophe. The GTP-cap size is thought to increase with the microtubule growth rate, presumably endowing fast-growing microtubules with enhanced stability. It is unknown what GTP-cap properties permit frequent microtubule catastrophe despite fast growth. Here, we investigate microtubules grown in vitro in the presence and absence of the microtubule polymerase XMAP215. Using EB1 as a GTP-cap marker, we find that GTP-cap size increases regardless of whether growth acceleration is achieved by increasing tubulin concentration or by XMAP215. In spite of the increased mean GTP-cap size, microtubules grown with XMAP215 display increased catastrophe frequency, in contrast to microtubules grown with more tubulin, for which catastrophe is abolished. However, microtubules polymerized with XMAP215 have large fluctuations in growth rate and EB1 intensity; display tapered and curled ends; and undergo catastrophe at faster growth rates and with higher EB1 end-localization. Our results underscore the role of growth irregularities in overall microtubule stability.Competing Interest StatementThe authors have declared no competing interest. ER -