PT  - JOURNAL ARTICLE
AU  - Li, Dapeng
AU  - Edwards, Robert J
AU  - Manne, Kartik
AU  - Martinez, David R.
AU  - Schäfer, Alexandra
AU  - Alam, S. Munir
AU  - Wiehe, Kevin
AU  - Lu, Xiaozhi
AU  - Parks, Robert
AU  - Sutherland, Laura L.
AU  - Oguin, Thomas H.
AU  - McDanal, Charlene
AU  - Perez, Lautaro G.
AU  - Mansouri, Katayoun
AU  - Gobeil, Sophie M. C.
AU  - Janowska, Katarzyna
AU  - Stalls, Victoria
AU  - Kopp, Megan
AU  - Cai, Fangping
AU  - Lee, Esther
AU  - Foulger, Andrew
AU  - Hernandez, Giovanna E.
AU  - Sanzone, Aja
AU  - Tilahun, Kedamawit
AU  - Jiang, Chuancang
AU  - Tse, Longping V.
AU  - Bock, Kevin W.
AU  - Minai, Mahnaz
AU  - Nagata, Bianca M.
AU  - Cronin, Kenneth
AU  - Gee-Lai, Victoria
AU  - Deyton, Margaret
AU  - Barr, Maggie
AU  - Von Holle, Tarra
AU  - Macintyre, Andrew N.
AU  - Stover, Erica
AU  - Feldman, Jared
AU  - Hauser, Blake M.
AU  - Caradonna, Timothy M.
AU  - Scobey, Trevor D.
AU  - Moody, M. Anthony
AU  - Cain, Derek W.
AU  - DeMarco, C. Todd
AU  - Denny, Thomas N.
AU  - Woods, Christopher W.
AU  - Petzold, Elizabeth W.
AU  - Schmidt, Aaron G.
AU  - Teng, I-Ting
AU  - Zhou, Tongqing
AU  - Kwong, Peter D.
AU  - Mascola, John R.
AU  - Graham, Barney S.
AU  - Moore, Ian N.
AU  - Seder, Robert
AU  - Andersen, Hanne
AU  - Lewis, Mark G.
AU  - Montefiori, David C.
AU  - Sempowski, Gregory D.
AU  - Baric, Ralph S.
AU  - Acharya, Priyamvada
AU  - Haynes, Barton F.
AU  - Saunders, Kevin O.
TI  - The functions of SARS-CoV-2 neutralizing and infection-enhancing antibodies in vitro and in mice and nonhuman primates
AID  - 10.1101/2020.12.31.424729
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2020.12.31.424729
4099  - http://biorxiv.org/content/early/2021/01/02/2020.12.31.424729.short
4100  - http://biorxiv.org/content/early/2021/01/02/2020.12.31.424729.full
AB  - SARS-CoV-2 neutralizing antibodies (NAbs) protect against COVID-19, making them a focus of vaccine design. A safety concern regarding SARS-CoV-2 antibodies is whether they mediate disease enhancement. Here, we isolated potent NAbs against the receptor-binding domain (RBD) and the N-terminal domain (NTD) of SARS-CoV-2 spike protein from individuals with acute or convalescent SARS-CoV-2 or a history of SARS-CoV-1 infection. Cryo-electron microscopy of RBD and NTD antibodies demonstrated function-specific modes of antibody binding. Select RBD NAbs also demonstrated Fc receptor-γ (FcγR)-mediated enhancement of virus infection in vitro, while five non-neutralizing NTD antibodies mediated FcγR-independent in vitro infection enhancement. However, both in vitro neutralizing and infection-enhancing RBD or infection-enhancing NTD antibodies protected from SARS-CoV-2 challenge in non-human primates and mice. One of 30 monkeys infused with enhancing antibodies had lung pathology and bronchoalveolar lavage cytokine evidence suggestive of enhanced disease. Thus, these in vitro assessments of enhanced antibody-mediated infection do not necessarily indicate biologically relevant in vivo infection enhancement.Competing Interest StatementThe authors have declared no competing interest.