RT Journal Article
SR Electronic
T1 Effect of sublethal prenatal endotoxaemia on murine placental transport systems and lipid homeostasis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.08.04.236745
DO 10.1101/2020.08.04.236745
A1 MW Reginatto
A1 KN Fontes
A1 VRS Monteiro
A1 NL Silva
A1 CBV Andrade
A1 HR Gomes
A1 GE Imperio
A1 FF Bloise
A1 G Kluck
A1 GC Atella
A1 SG Matthews
A1 E Bloise
A1 TM Ortiga-Carvalho
YR 2021
UL http://biorxiv.org/content/early/2021/01/07/2020.08.04.236745.abstract
AB Based on accumulating evidence, infection alters the expression of transporters that mediate the placental exchange of xenobiotics, lipids and cytokines. We hypothesized that lipopolysaccharide (LPS) modifies the expression of placental transport systems and lipid homeostasis. LPS (150 μg/kg; i.p.) treatments were administered for 4 h or 24 h, animals were euthanized at gestational days (GD) 15.5 or 18.5, and maternal blood, foetuses and placentae were collected. Increased rates of foetal demise were observed at GD15.5 following LPS treatment, whereas at GD18.5, high rates of early labour occurred and were associated with distinct proinflammatory responses. LPS decreased placental fatty acid binding protein associated to plasma membrane (Fabppm) expression at GD15.5 (LPS-4 h), increased Fat/Cd36 expression at GD18.5 (LPS-4 h) and reduced Abcb1b, Abcc2 and Abcc5 expression at GD18.5 (LPS-24 h). At the protein level, breast cancer-related protein (BCRP) and Abcg1 levels were decreased in the labyrinth zone (Lz) at both time points, whereas P-glycoprotein (P-gp) levels in the Lz were decreased at GD18.5. Specific maternal plasma and placental changes in triacylglycerol, free fatty acid, cholesterol, cholesterol ester and monoacylglycerol levels were detected in a gestational age-dependent manner. In conclusion, LPS was associated with increased foetal death and early labour by decreasing placental ABC transporter expression and altering maternal plasma and placental lipid homeostasis. These changes likely modify foetal xenobiotic exposure and placental lipid exchange in cases of bacterial infection.Competing Interest StatementThe authors have declared no competing interest.